ABSTRACT
<p><b>OBJECTIVE</b>To investigate the polymorphism of mitochondrial DNA region V in Bouyei people and Miao people living in Guizhou province of China.</p><p><b>METHODS</b>Polymerase chain reaction and direct sequencing were used in the study.</p><p><b>RESULTS</b>Only two kinds of polymorphism were found in Bouyei and Miao people. One was standard pattern, the other short pattern. And the frequencies of short pattern(9 bp deletion) in Bouyei and Miao people were 31.1% and 33.3% respectively.</p><p><b>CONCLUSION</b>The polymorphisms of mitochondrial DNA region V in Bouyei people and Miao people living in Guizhou province of China are similar, but they are different from those in other people.</p>
Subject(s)
Female , Humans , Male , Base Sequence , China , DNA, Mitochondrial , Chemistry , Genetics , Gene Frequency , Polymorphism, Genetic , Genetics , Sequence Analysis, DNA , Sequence DeletionABSTRACT
Objective:To study the gene expression spectrum of Th cell immune response in acute and chronic toxoplasma infection in BALB/c mice.Methods:BALB/c mice were infected with toxoplasma tachyzoite (1?104/m,0.5 ml) by intraperitoneal injection.Two mice were killed at 6th and 12th day postinjection.The immune response gene expressing levels in mice spleen cells were detected with 128 kind gene microarray chips.Partial of the results were determined with Real time-PCR.Results:There were 20 genes changed after toxoplasma infection.Eight of them (Gata-3,Ccr3,Ccr4,Bcl6,Nfatc1,CD80,Fos,CD69) were regulated upward and twelve genes (T-bet,CIITA,Irf1,Mapk9,Nfatc3,Fasl,Tyk2,Lat,Mapk10,Socs3,Socs6 and Yy1) were regulated downward.On the whole,expression of Th2 response genes presented a up trend,and Th1 response genes presented a down trend.The results of Real time-PCR were accordant with microarray in the expression of Gata-3 and T-bet gene.Conclusion:According to the Th cell immune response gene expression spectrum,Th1 immune response is dominant in the acute infention when mice are infected by toxoplasma.Then,it is biased toward Th2 immune response.
ABSTRACT
Objective:To study single nucleotide polymorphisms in the promoter region of TNF-? gene in Chinese Han,Miao and Buyi population in Guizhou province.Methods:The five polymorphism sites at-1031,-863,-857,-308,-238 in TNF-? gene promoter region were detected using PCR-RFLP and SSP-PCR.Results:The allele frequencies of TNF-? gene in promoter region in these three main ethons of population were observed.In Chinese Han population,the-238 G was 96% and-238 A was 4%.Inaddition,-308 G was 92% and-308 A was 8%,-857 C was 65% and-857 T was 35%,-863 C was 80% and-863 A was 20%,-1031 T was 48% and-1031 C was 52%;In Miao people,the frequency at the loci above were 15.0%,8.0%,26.0%,4.0% and 50.0%;And the frequency of these five loci above were 14.0%,6.0%,11.0%,16.0% and 50.0% in Buyi population.Conclusion:Single nucleotide polymorphisms of TNF-? gene exhibits some specific characters in Chinese Han and Miao and Buyi population in Guizhou province.
ABSTRACT
Objective:To explore the polarization of Th1 and Th2 immune response and the changes of transcription factor(T-bet and GATA-3) in BALB/c mice infected with toxoplasma gondii.Methods:Sixty BALB/c mice were infected with toxoplasma tachyzoite(1?104/m,0.5 ml) by intraperitoneal injection and thirty BALB/c mice only received an injection of same amount of normal saline as control.After injection,two mice of infection group and one mouse of control group were killed in odd number days.Then,the serum IFN-? and IL-4 were measured by ELISA,and the mRNA expression of T-bet and GATA-3 in mice spleens were detected by Real-Time RT-PCR.Results:In the infection group of mice,the IFN-? was remarkable increased at the fourth day and kept the peak from the 5th day to 7th day.Then,it decreased to normal level at 9th day postinjection;but IL-4 was remarkable increased at the 8th day and reached to the peak at 9th day.Then it decreased to normal level at 15th day postinjection.The mRNA of T-bet expression was increased at the third day and kept the peak from the 5th day to 7th day.Then,it decreased to normal level at 9th day postinjection.The mRNA of GATA-3 expression was increased at the 7th day and rised to the peak at 11th day.Then,it decreased to normal level at 13th day postinjection.There were not obvious changes of IFN-? and IL-4 or T-bet and GATA-3 in normal mice.Conclusion:The Th1 immune response is dominant in acute infection when mice are infected by toxoplasma.Then it biases toward Th2 response at 9th day to 13th day postinfection.The shift of Th1/Th2 polarization influences the finale of toxoplasma infection.